3RAD data of sea turtles hybrids from Abrolhos Archipelago - SRA

SRX8613830: 3RAD data of sea turtles hybrids from Abrolhos Archipelago
1 ILLUMINA (Illumina HiSeq 4000) run: 2.3M spots, 675.5M bases, 314.5Mb downloads

Design: We used the 3RAD protocol as described by Bayona-Vsquez et al. to analyze hatchlings from six different nests collected at Abrolhos. Briefly, we digested 100 ng of genomic DNA with the enzymes MseI, EcoRI and CviQI at 10 U/L and the specific combination of adapters for each sample for one hour at 37C. We proceeded with the ligation of adapters containing in-line barcodes on both P5 and P7 adapters, immediately after the digestion. Barcoded samples were equimolarly pooled and cleaned with 0.8X CleanPCR magnetic beads (GC biotech). Subsequently, we performed the size selection of fragments between 350 and 450 bp with Blue Pippin using a 1.5% cassette and the R2 marker. The libraries were submitted to a single-cycle PCR to add the Tru5-8N primer, followed by a ten-cycle indexing PCR, in which we used a P5 short primer and P7 indexing primer to complete the library construction. The final library was characterized with qPCR using the KAPA Library Quantification Kit (Kapa Biosystems) and checked with the Agilent 2100 Bioanalyzer High Sensitivity DNA. The fragments were sequenced using 150 bp paired end reads in one partial lane of the HiSeq 4000 platform (Illumina) with the TruSeq 300-cycle Kit, aiming at a minimum of 20x coverage per individual sequenced.

Submitted by: Federal University of Minas Gerais

Study: 3RAD data for sea turtles from Abrolhos

PRJNA641778 • SRP268741 • All experiments • All runs

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Data generated to investigate the hybridization between sea turtles species in Abrolhos Archipelago, Brazil. The data showed that there are first-generation hybrid females nesting in Abrolhos, resulting from crossings between hawksbill males (Eretmochelys imbricata) and loggerhead females (Loggerhead turtle), and backcrossed hatchlings from both parental species. The reproductive output data of Abrolhos nests suggests a disadvantage of hybrids when compared to loggerheads. For the first time, we have shown the association between hybridization and low reproductive success, which may represent a threat to sea turtle conservation.

Sample:

SAMN15366341 • SRS6903028 • All experiments • All runs

Organism: Caretta caretta x Eretmochelys imbricata

Library:

Name: 3RADseaTurtles15

Instrument: Illumina HiSeq 4000

Strategy: RAD-Seq

Source: GENOMIC

Selection: Restriction Digest

Layout: PAIRED

Runs: 1 run, 2.3M spots, 675.5M bases, 314.5Mb

Run	# of Spots	# of Bases	Size	Published
SRR12087521	2,308,133	675.5M	314.5Mb	2020-06-26

ID:: 11196011

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